Every year, insects like the cotton bollworm destroy more than 20 percent of the world’s crops. Farmers fight back by using pesticides, but some are harmful to our health, and many damage surrounding ecosystems. Clearly, more environmentally friendly approaches to pest control are needed, and there’s one solution that could be about to hit the big time: targeting these pests’ sex drives. Female insects can attract partners in complete darkness without any audible signal, and over hundreds of meters—sometimes over a kilometer—using sex pheromones. Males track the smell of these chemical signals and mate with the females they’re led to, who then lay eggs that hatch into hungry larvae. It’s an incredible chemical power—and one that can be exploited. “We can apply artificial pheromone compounds into the field, which will be released everywhere in the air and cover the original signal from the real female,” says Hong-Lei Wang, a researcher in the pheromone group at Lund University in Sweden. This blanket cover of the sex scent makes it harder for males to find females and mate, he explains, and so the insect population falls, meaning fewer pests in the area to cause crop damage. Farmers have been using artificial pheromones this way for decades—but up until now, costs have limited how widely they’re used. Creating artificial pheromones has been pretty expensive, so it’s only made economic sense to use them to protect high-value crops, such as fruits. But now Wang and his colleagues have uncovered a way to affordably and sustainably produce pheromones that attract pests that eat cheaper crops, such as cabbage and beans, opening the door for pheromone-based pest control to be used more widely. In a paper published in Nature Sustainability, the group showed how to make significant amounts of two important moth pheromones from an oilseed plant. They then demonstrated that the artificial pheromones worked well in mass trapping experiments as well as in disrupting the mating of the cotton bollworm. “The first thing was to work out the pathways for pheromone production in the insects,” says Christer Löfstedt, coauthor of the study and professor of functional zoology at Lund. This was done by looking at the insects’ genes to find the ones that control production of the desired sex pheromone. Then the group introduced these genes into a different biological platform—which in this study was the oilseed crop, though yeast would also work—so that it would make the sex pheromone at scale. Finally, the compound was isolated and purified to get it ready for testing in the field. For the experiment, the new plant-derived pheromones were tested in the field using pheromone dispensers, with their effectiveness being compared against conventional synthetic pheromones. Both versions were equally effective in trapping insects and disrupting their mating, shown as a decline of the insects’ male population over time. This proved the plant-derived pheromone to be a viable alternative to the artificial pheromones traditionally used. The beauty with pheromones is that they don’t kill insects, but instead just influence their behavior. This makes them more environmentally friendly than pesticides, which often wipe out many species that aren’t the target, explains Srinivasan Ramasamy, a lead entomologist at the nonprofit World Vegetable Center. Pesticides are commonly found in soil, air, water, and on non-target organisms in our ecosystems. They can harm plants and animals, ranging from beneficial soil microorganisms and insects—such as bees, pollinators, spiders, and mites—to plants, fish, birds, and other wildlife. Insects also distinguish whether a pheromone signal is from their own species, meaning you can target the life cycle of specific pests while leaving other insects unaffected. “Specificity is a very clear feature of the pheromones system,” says Wang. It’s not only good news for a lot of bugs out there, but also farmers and consumers. Insect pheromones are not toxic to humans, which is a real benefit for agricultural workers. “It has been demonstrated that conventional insecticides have a negative impact on, for instance, the fertility of farm workers,” explains Löfstedt. Some pesticides target bodily systems in insects that share physiological similarities with those in humans, such as neurotransmitters. “When you are exposed to the pesticides, they can impact your physiology,” says Ramasamy. And importantly, insects don’t evolve resistance to their own communication signals, says Löfstedt, whereas they do to pesticides. Repeated use of a pesticide family commonly leads to insects adapting and no longer responding to those same chemicals, meaning higher and higher doses are needed to have an effect—with dire environmental consequences. But even if the technique were scaled up, there will be limits to how it can be used. Cooperation between neighboring farmers is also key, as mating disruption works best if large areas are treated. Female pests may mate outside the fields, and travel later and lay eggs. “It’s usually not very successful to treat a home garden with pheromones,” says Löfstedt. There’s regulation to overcome too. The fact that it’s a genetically modified (GMO) technology could prove a major roadblock and also drive up costs, Ramasamy warns. “In most countries, the production of GMOs may not be very expensive, but the regulatory dossier they have to undergo needs a lot of investment,” he says. In fact, stringent rules on using GMOs in Europe could mean the continent misses out on these products, Löfstedt says. Finally, there are biological limits to how widely pheromones can be used as well—they’re not an option for all pests. “Not all insects have sex pheromones,” Löfstedt explains, and some that do produce complex compounds that are not straightforward to bioengineer. In some cases, the sex pheromone is made up of two or three compounds. “Then you need to have two or three genetically modified plants to be produced and put under the regulatory mechanism,” Ramasamy says. The reality is that for agriculture to be sustainable, it may need to involve a mix of pest control techniques—such as botanical pesticides made from fungi or viruses and pest-resistant plant varieties, used together with engineered pheromones. But for now the pest control competition is fierce: Pesticides are easily available, easy to apply, take care of multiple insects, and are relatively cheap. “If we go with five different components in an integrated pest-management program, then the farmers are going to say, ‘No, sorry,’” says Ramasamy. The key will be to find a sustainable solution that also keeps things simple, he suggests. Löfstedt is adamant, though, that plant-grown pheromones can be a big part of pest control in the future. “It’s green chemistry, driven by solar energy,” he says—yet another plus point. If we want to be sustainable and protect wildlife, crops, and human health, then we need to find pest control methods like this that can realistically be scaled up so that pesticide use falls—and make sure it happens. It’s up to us to “use biological knowledge, and the different control methods, in a biologically sensible way.”
